I'd suggest British actress Rebecca Hall for the role:
She was awesome in The Town. Looking forward to Everything Must Go. She is definitely a rising star and a good fit for this role.
Originally Posted by Golgo-13
I think the actress that plays Jessica the baby vamp on HBO's True Blood would make a good Spider-Woman. She's not super model hot when she has on regular clothes, just cute, but still has a great body under there....
Deborah Ann Woll is definitely hot enough! Not sure about her acting chops.
Originally Posted by cherokeesam
Uma Thurman? Kate Beckinsale? Charlize Theron? Sandra Bullock? Linda Hamilton? Lynda Carter? Demi Moore? Lucy Liu? Sigourney Weaver? Cameron Diaz? They all made bank as action heroines.
Originally Posted by HR-PUFF&STUFF
In Plan Sight
La Femme Nikita
Law & Order SVU (it may not be a female driven show, but it has strong females on the show)
I think The Long Kiss Goodnight is missing from these lists. Charlie was about as kickass as a heroine gets.