Alfred: John Hurt.
Simon Pegg as Riddler.
Scarlett Johanson as Harley.
David Finlay as Mad Hatter.
Michael Rouke as Croc.
Mary Elizabeth Winstead as Catwoman/Selina Kyle.
Kevin Costner as Kirk Langstorm.
Adrien Brody as Professor Achiles Milo.
Guy Pierce as BlackMask.
Rachel Weisz as Red-Claw.
Ben Kingsly or Malcom Mcdowell as Hugo Strange.
Kate Beckinsale as Zantanna.
Doug Jones as Clayface/Basil Carlo.
Gerald Butler as Maximillion Zeus.
Philip Seymore Hoffman as Penguin/Oswold Copplepot.
Isla Fisher as Poison Ivy.
Kevin Spacey as Mr. Freeze.
Jon Voight as Rupert Thorn.
Hugh Jackman as Anthony Romanus.
William H. Macy as Karl Rossom.
Jamie Lee Curtis as Dr. Leslie Thompkins.
David Cronenberg (he has done some acting chops in films especially Jason X and Nightbreed) or William Dafoe as Dr. Emile Dorian.
Robby Benson (Beast on Beauty and The Beast) or James Avery (Uncle Phil on Fresh Prince with Shredder in the old turtles toon and Fang on Fist of the North Star) as the voice of Tygrus.
Debbie Carrington (Midget actress from Garbage Pail Kids movie, Total Recall and others) as Baby Doll.
Simon Pegg as Riddler.
Scarlett Johanson as Harley.
David Finlay as Mad Hatter.
Michael Rouke as Croc.
Mary Elizabeth Winstead as Catwoman/Selina Kyle.
Kevin Costner as Kirk Langstorm.
Adrien Brody as Professor Achiles Milo.
Guy Pierce as BlackMask.
Rachel Weisz as Red-Claw.
Ben Kingsly or Malcom Mcdowell as Hugo Strange.
Kate Beckinsale as Zantanna.
Doug Jones as Clayface/Basil Carlo.
Gerald Butler as Maximillion Zeus.
Philip Seymore Hoffman as Penguin/Oswold Copplepot.
Isla Fisher as Poison Ivy.
Kevin Spacey as Mr. Freeze.
Jon Voight as Rupert Thorn.
Hugh Jackman as Anthony Romanus.
William H. Macy as Karl Rossom.
Jamie Lee Curtis as Dr. Leslie Thompkins.
David Cronenberg (he has done some acting chops in films especially Jason X and Nightbreed) or William Dafoe as Dr. Emile Dorian.
Robby Benson (Beast on Beauty and The Beast) or James Avery (Uncle Phil on Fresh Prince with Shredder in the old turtles toon and Fang on Fist of the North Star) as the voice of Tygrus.
Debbie Carrington (Midget actress from Garbage Pail Kids movie, Total Recall and others) as Baby Doll.