Hah, I knew I should have searched around for a topic like this! I was just about to start one when I remembered it
As we all know everyone in this show with a name is from somewhere in the comics. I was watching The Uncertainty Principle and when the credits came on I noticed Steve Blum also voiced a character named Dilbert Trilby. I assume that he is that news reporter. I went and did my research and in the comics Dilbert Trilby is the Daily Bugle's obituary writer who first appeared in Spider-Man Unlimited #3 in 1993.
Also we've seen Seymour O'Reilly in the show as well He the tanned kid who has curly hair and glasses who was trying to get Mark Allan on the gambling path again in First Steps. In the series, he's appeared for sure in First Steps and in Identity Crisis and had a small speaking part in both of episodes. In comics he was a relatively minor character who was just one of the "in crowd". He was also friends with Peter at one point in time but they started to drift apart as Peter was more interested in science than social gatherings. He first appeared in Amazing Fantasy #15 in 1962. He's currently dead I believe, killed by the Angelo Fortunado version of Venom.
Next up we have Officer Vincent (Vin) Gonzales, who is a cop who showed up in Probable Cause for sure in the cop car with his partner Alan O'Neal, taking Mark Allan and Mary Jane Watson on their "ride along". I think he was in Natural Selection as well as the cop who was shooting at the Lizard in the subway, (although on TV he did not shoot, he simply just aimed, damn censors) so basically he's the only Latino cop we've seen so far in the show. Anyway in the comics he's actually Peter Parker's roommate and of course part of the NYPD. Apparently he's also Pete's romantic rival (for Carlie Cooper) since One More Day's divorce settlement. He first appeared in 2007, one of you guys will have to help me out on the comic book as I'm not a Spidey buff (my buddy is though) I'm more of a Batman buff. However I must say this show has definitely got me interested.
Anyone find anyone else?