When Norman Osborn hears that hes facing financial ruin, he becomes Green Goblin and encounters Spider-Man for the first time in battle. Spider-Man then gets the symbiote and takes it to have it looked at by Dr. Connors, who informs him what it is and what its intentions are. Despite Connors warning, Peter keeps the symbiote and starts acting differently, much to Gwens dismay. Peter and Gwen begin drifting apart throughout the film.
Eddie Brock (Jensen Ackles), introduced as a journalist for the Daily Bugle in Amazing Spider-Man 2, finds someone actually confessing to be Green Goblin and writes an article revealing the mans identity, which is published by the Bugle. However, Spider-Man reveals that Brock has been interviewing a compulsive confessor. Brock is fired from the Bugle and blames Spider-Man for his downfall.
Green Goblin abducts Gwen and lures Spider-Man to a bridge. The two fight, and Green Goblin hurls Gwen off the bridge. Spider-Man shoots a strand of web at her leg and catches her. As he pulls her up, he thinks hes saved her, but soon realizes shes dead. Green Goblin escapes, and Spider-Man tracks him down to a warehouse, where Peter violently beats Norman to a pulp, but cant bring himself to kill him and realizes that the symbiote is beginning to take over. Norman uses the opportunity to send his glider to impale Peter from behind. Warned by his spider sense, Peter jumps out of the way just in time, and the glider instead impales Norman and kills him.
Spider-Man swings to a nearby church and removes the symbiote, using the sound of the church bell to weaken it. Peter, weak as well from the removal, starts walking home.
Peter, back at home and devastated, encounters an equally saddened and shocked Mary Jane, whos lost her friend, and the two comfort each other.
Meanwhile, Brock is seen entering the same church Spider-Man was at and kneels at the altar. Brock asks for forgiveness for contemplating suicide, unaware that above him is the symbiote moving quickly toward him. Brock is engulfed by the symbiote and starts transforming into a bigger, black Spider-Man. A giant white spider emblem appears on his back and chest, along with a white square on each hand. A large pair of white eyes are formed, and razor sharp teeth are shown. Venom looks up at the camera and screams as the screen goes black.