At the beginning of the game,Gwen asks Peter to come visit her at work. Gwen has scored the prestigious position of chief intern at Oscorp, which grants her access to some of the company's secure labs. Gwen doesnt like what she sees. In the wake of Dr. Connors' meltdown, osCorp inherited date on cross-species genetics. The company hired Alistair Smythe to help sort through the mess of notes Connors left behind. Gwen believes that OsCorp has the genius and the resources to turn Connors' work into a blessing, but she suspects it is being used for a sinister purpose. She asks Peter to help uncover the truth.
As the two kids walk through the halls of OsCorp, they spy a number of full-grown cross-species test subjects. Spider-Man fans might recognize many of these tests subjects as Spider-Man's villians like Vermin, Rhino, and Scorpia. There were also references to Felicia Hardy, Iguana, and Morbius during the opening hours. As Peter skulks deeper into the complex, many of the cross-species become agitated---Hooting, hollering, and beating their fists against the glass cages. They seem to be reacting to another cross-species. They are reacting to Spider-Man.
As Gwen and Peter work their way through OsCorp facility, they notice a few scientists preparing a test subject for transport. As Peter approaches one test subject, the beast becomes highly agited and pounds against the glass enclosure. As if on cue, dozens of test subjects begin to howl and tear free of their bonds. OsCorp decends into chaos.