Cmdr. John Shepard - Matthew Goode, Matthew Fox, or Guy Pearce
Saren Arterius - Fred Tatasciore, Sam Neill, or Jeremy Irons, with motion capture and CGI
Matriarch Benezia - Marina Sirtis
Admr. David Anderson - Keith David, Laurence Fishburne, or Giancarlo Esposito
Flt.Lt. Jeff "Joker" Moreau - Seth Green, Danny Masterson, or Seann William Scott
Dr. Karin Chakwas - Carolyn Seymour, Emma Thompson, or Helen McCrory
Admr. Steven Hackett - Lance Henriksen, Stephen McHattie, or Michael Ironside
Councilor Donnel Udina - John Noble, or Ben Kingsley
Illusive Man - George Clooney, Liam Neeson, or Bruce Greenwood
Kai Leng - Dan Southworth, or Byung-Hun Lee
Liara T'Soni - Ali Hillis, Jennifer Lawrence, or Gemma Arterton, with prosthetic makeup
Tali'Zorah nar Rayya - Ash Sroka, Summer Glau, or Emily Blunt, with an opaque mask
Garrus Vakarian - Brandon Keener, with motion capture + CGI and/or prosthetics
Urdnot Wrex - Steven Barr, with motion capture + CGI and/or prosthetics
Miranda Lawson - Yvonne Strahovski
Thane Krios - Doug Jones
Jacob Taylor - Michael Jai White, Chiwetel Ejiofor, or Isaiah Mustafa
Kasumi Goto - Kim Hoy
Jack - Katee Sackhoff
Legion - D.C. Douglas, with motion capture + CGI