For me, the voice I hear is dependant on the era of the comics, due to the change in art styles, mood, whatever.
60s comics: Paul Soles
70s comics: Nicholas Hammond
80s comics: Chris Barnes
90s comics: Barnes (until Bagley leaves) then, Rino Romano
USM: Neil Patrick Harris
I haven't seen enough of the 80's cartoons to remember their voices, which is a shame, I've seen enough of them to remember how good the shows were (and the voices, too), but not extensively enough to remember the voice casts. They were never on when I was younger, and ABC Family runs them at poor times for me to catch. If Disney would release the damn shows on DVD, I'd be able to see them in detail.
And, might I add, that I don't think there's ever been a bad Spider-Man voice actor. I even like Tobey Maguire, though he's far from #1 on my list...