UV light cause DNA breaks in both strands at multiple random places. This is proven, we even have a little machine in the lab that looks like a vintage toaster oven that emits UV at specific doses. When we want to test how a cell line can repair UV damage, we stick in a plate of cells, set it, and then turn it on and off it zaps.
Strong UV does a good job of sterilizing things. You might have seen toothbrush sanitizers or water purifiers that utilize UV light. The UV in them is so strong, it causes such massive DNA breaks that the cells die in short order. We have a UV light in our tissue culture hood where we work with cells. When we're not using it, the UV light is on, to kill bacteria and other things (including our own DNA
) we don't want contaminating our samples. Once I asked my boss how strong it was, and he said it would give me a sunburn in 5 minutes.
The new hoods have a safety mechanism so the UV light can't come on unless the hood is closed, in case some people are stupid and turn the UV light on while other people are working nearby.
I couldn't give you a percentage. It really depends on where the DNA damage occurs (since it is completely random) and how that person's cells repair it. But obviously the more it happens, the odds are greater that a bad mutation occurs. It's also hard to study because the damage is random and happens in multiple places. I think people just use UV damage to study the overall ability of a cell line (cells with a known specific mutation) to repair such damage. It's not the best route to study the mechanism a cell would actually use in order to repair the DNA, because DNA is so long and convoluted. What my lab does is create a break in a specific point (so we know where and how to look) and see how the damage is repaired. We just use UV as another comparison tool.