When I was kid Rosemary's Baby was one of most disturbing things I saw. But I dont know now. To me disturbing has completely different meaning from usual violence, torture or something gross to watch.
Also Bone Tomahawk has one of the most upsetting sequences I think I've ever seen.
Men Behind The Sun
Guinea Pig
Nekromantic
...Human Centipede is a walk in the park.
And then of course, there's this:
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Truly the most disturbing movie ever made.
An American Crime (one of the most despairing films I have ever seen)