I think Spiderman would have problems with Spot. Spot is really out of Spidey's league, don't let the fear rating fool you. The fear rating is based on how much effect the villain has on Spidey life. Because the Spot rarely makes an appearance or rarely have confrontations with Spidey, his fear rating is low. It has nothing to do with how bad arse the villain is, nor how powerful the villain is. It's basically the amount of confrontations and effect the villain has on Spidey's livelihood.
In a live action movie, Spot would be to much for Spidey. How would he deal/fight him. Spidey will have to try to out smart him.... a scientist. That will be hard to do, unless he talk him down like he did Doc Ock.