So many great ones that folks have mentioned but the defining scene for me was the rubble scene. It was reminiscent of the "he's just a kid" scene in SM2 and that one still makes me teary eyed. But this one is more impressive because it's all on Tom to create such a compelling moment and he delivered. There are quite a few moments in this movie, though, that reminds the audience that despite Peter having super powers, he is still just a kid and it was all in actions...no dialogue needed. That's one of the aspects of this movie that now makes it superior to every spidey film that came before, imo.