I thought Spectacular didn't do a bad job. Memory is fuzzy and I'll probably have to watch it again, but every time some screwup happened in Eddie's life, Peter and Spider-Man where somehow involved. Sure, all those times Peter tried to help, but he couldn't say that it was him who fought and gave Connors the gene-cleanser. So when you get that Lizard vs Spider-Man picture in the Daily Bugle, Peter comes across like a selfish jerk, who abandoned his friends for some money. And there were several moments like that. Than you have the symbiote who reveals to him that Spider-Man and Peter are the same, and increases the already existing hate for him.
There was actually a good build-up to give Eddie a proper reason to hate Spidey/Peter, instead of hating him just because he caught the real murderer.