(the Haitian voodoo priestess with ressurection abilities who controlled lizard in the comics) and Kraven who after Spidey accidentally kills him Calypso turn to the lizard as her muscle to get revenge, plus Calypso will know of the bond that Peter and Dr. Connors share and use Peter's hesitance to consecutively defeat him and try to kill him
Peter's conflict-facing personal temptation, and finding who he is- as Calypso will use temptation to lure the Lizard (restoration of his arm), and later Peter (restoration of Uncle Ben), ulimately peter contemplate his morals, whether to stop Callisto, or let her bring back uncle Ben, plus his actions will define Dr. Connors chances of restoring his arm
--throughout the film, Calypso could torment him with psychic images of uncle Ben, or make Peter hear his voice every so often,
--a climactic scene could be after spidey defeats the lizard Calypso confronts him, but
in the illusion of uncle Ben
Calypso could be very devious and manipulative, dangling uncle Ben and Dr. Connors in front of Peter to alter his moral obligations