In Sub-Zero, Mr. Freeze was not harvesting women-- he merely kidnapped Barbra Gordon because she had Nora's rare blood type (O Negative). He had no intention of harming Barbra during the whole ordeal, even though he did resort to kidnapping.
I hope that they use Mr. Freeze the way they did in the animated series, where he is a truly tragic character who the audience actually feels a lot of pity for. They tried quite miserably to bring some of that to his character in Batman and Robin, and no-one could take it seriously because the whole movie was so campy and Freeze was played as so goofy by Schwarzenegger. Hopefully, someone has the guts to do Freeze justice.
If they wanted to use multiple villains in a movie with Freeze, they could quite comfortably replace Ferris Boyle with The Penguin, and set up Oswald Cobblepot as being a powerful and corrupt CEO-- a true "anti-Bruce Wayne." Cobblepot would be responsible for the creation of Mr. Freeze because he tried to pull the plug on Victor Fries' research, which would kill Nora. Victor would attempt to blackmail him since he has learned about his illegal activities. Cobblepot tries to kill Fries and destroys his lab, leaving him for dead, but a cryogenic chemical spill allows Fries to survive-- at the price of having to wear a refrigeration suit for the rest of his life.