sure... why not?
after thinking about it....most. excluding his top 2 (ock and GG) have all been used multiple times though we've never had a classic goblin in anything but the movie-verse (including friend or foe)
so including movies (N64/PS1 to present consoles):
-doc ock
-green goblin
-hobgoblin (if you want to count SD)
-venom
-electro
-sandman
-kraven
-calypso
-lizard
-mysterio
-rhino
-shocker
-vulture
-morbius
-kingpin
-juggernaut
-carnage
-scorpion
-black cat
-hammer head
-Prowler
-Beetle
-Silversable
-Shriek
-Chtylok (web of shadows PS2)
-Jackal (WoS PS2)
-Spider-woman (web of shadows PS2)
have all been used in a video game so far... though some of these once... pretty much everyone short of Man-Wolf, Tarantula, Demogoblin, Jackal, Spot, Jack-o-lantern, etc... have been "fightable, playable, or assisting ally" in a spider-man game over the least 11 years... but, ignoring the movies, friend or foe, and alternate universes.... only a small handful of these have ever been used... adding to that, some havent been used for around a decade. below are the amounts of times a "616" known spider-man villain has been used as a boss
those are..
-doc ock 1x (spider-man n64-ps)
-green goblin 0x
-hobgoblin 0x
-venom 2x (spider-man n64-ps/WoS)
-electro 2x (enter electro/Wos)
-sandman 2x (enter electro/SD)
-kraven 2x (WoS (ps2)/SD)
-calypso 0x
-lizard 2x (spider-man n64-ps/enter electro)
-mysterio 3x (Spider-man n64-ps/WoS(ps2)/SD)
-rhino 2x (Spider-man n64-ps/WoS)
-shocker 2x (enter electro/WoS(ps2))
-vulture 1x (WoS)
-morbius 0x
-kingpin 0x
-juggernaut 1x (SD)
-carnage 1x(spider-man n64-ps)
-scorpion 1x (spider-man n64-ps)
-hammer head 0x
-Beetle 0x
-Shriek 0x
this means..... it's about time imo mysterio sits this one out... and also... do to all the non 616 games.... i say it's time the symbiotes, scorpion, rhino, and shocker all sit this next game out too