Yes he did. Like I said in my last post, one of the earliest Spidey comics dealt with this. ASM#10.
And there are plenty of other instances in the comics as well.
Tragic event? No. But when you've got a huge chip on your shoulder, from a character standpoint, it's good to have a reason, and even better if it's one that can at least be sympathized with.
Yes he does. But it's because, as his reasoning in ASM #10 and other issues points out, he does so because he wants to knock Spider-Man down so he can feel better about himself. He also believes (at last publicly) that Spider-Man thinks he is better than everyone else and his actions are nothing more than him flaunting his superiority and that his battles are either staged, or that the bad guys wouldn't exist if Spider-Man wasn't around.