I would avoid citing "because it happened in the comics"
norman osborn impregnated gwen in the comics
spider-man had a spider-mobile at one point
better to argue the merits of pantsing rhino in terms of humour, which is subjective, and tone, which we can't really gauge.
if spider-man is constantly one-upping aleksei in the chase scene, and frustrating him more and more, then topping that all off with a pantsing could definitely work. a proverbial cherry on the cake that is aleksei's humiliation.
i think it will also depend on how much banter they have beforehand. it looks like there is at least some. it less weird to pants someone you have somewhat of a relationship with, than a complete stranger.
if there is a decent back and forth between spider-man and aleksei and/or a badgering element from the former in general, then the pantsing can work and not feel forced or out of place.